Phytochemistry is a study of phytochemicals which are the the secondary metabolic substances found in plants.They play an essential role to defend themselves from various pathogenic microbes which can be sometimes harmful. Phytochemicals in fruits may reduce the risk of cancer possibility due to presence of phenols, anti-oxidants and anti-Inflammatory substances.Antioxidants have already been found in plants and their supplements. Phenolics are secondary plant metabolites ranging from simple structure with one aromatic ring to complex structures such as tannins and lignin. The recreation towards phenolic compounds, particularly flavonoids and tannins have considerably increased.Extracts of fruits, vegetables, cereals and other plant materials rich in phenolics are increasingly of interest in food industry because they retard oxidative degradation of lipids and thereby improve the quality and nutritional values food.the total phenotypic content in the seeds of citrus fruits should be acquired by performing some tests . Materials and Methods:-The seeds of various citrus fruits have been collected and dried for a week. The dried seeds are crushed and powdered. This powder is now mixed with water and a extract is formed. This extract is used for the tests in order to determine the phytochemical analysis and phenolic content of the citrus fruit seeds. This study reveals the total phenolic content and phytochemical analysis of various citrus fruit seeds.Seeds of orange and lemon were taken for this research.The sample solution of both the fruits has been made.Tests done for phytochemical analysis are mentioned below:-1) TEST FOR PHYLOBATANNINS :-Take 1ml of the sample solution in a test tube and add 1ml of hydrochloric acid( HCL) and mix it well. Put it for a water bath for 3 minutes. Formation of red coloured precipitate confirms a positive result.2) TEST FOR CARBOHYDRATES :-a) FEHLING’S TEST: Take 1ml of solution in a test tube and add 1ml of Fehling solution A and B. Mix it well and put it for a water bath for about 3 minutes and remove it. Formation of red coloured precipitate confirms a positive result.b) BENEDICT’S TEST :-Take 1ml of sample solution in a test tube and add 1ml of Benedicts reagent. Mix it well and put it for a water bath for about 3minutes and remove it. Formation of red coloured precipitate confirms a positive result.3) TEST FOR FLAVANOIDS :-Take 1ml of sample solution in a test tube and add 2% of 2ml of sodium hydroxide( NaOH). Mix it well. The solution turns into a intensive yellow colour which is turned into colourless when 2 drops of diluted hydrochloric acid(HCL) is added to the solution.The result indicates the presence of flavanoids. 4) TEST FOR ALKALOIDS:-Take 1ml of sample solution in a test tube and add 3ml of hexane to it. And shake it well. Add 1ml of 2% of hydrochloric acid(HCL) and add few drops of picric acid to the solution.And mix it well. Formation of yellow coloured precipitate confirms a positive result.5) TEST FOR TERPENOIDS :-Take 1ml of sample solution in a test tube and add 2ml of chloroform. Mix it well and put it for a water bath for about 3minutes and remove it. Add 2ml of concentrated sulfuric acid( H2SO4).Formation of grey coloured precipitate confirms a positive result.Test for phenolic content is mentioned below:- The total phenolic content of both orange and lemon seeds was calculated using Folin ciocaletu’s reagent. The seeds of citrus fruits were dried and crushed into power. The powder is then mixed with water to form a solution. The sample solution of both the fruits has been made.Take 3 dry test tubes. In the first test tube add 1ml of total protein reagent and add 20microlitre of standard reagent. In the second and third test tube take 1ml of total protein reagent in each of them and add the both the sample solutions in the respective test tube. All the test tubes were shaken well and then kept for incubation for 10minutes at room temperature. For the qualitative analysis of total phenolic content, caloriemetre was used. First an empty dirt test tube was kept in the calorie meter and the meter was adjusted to zero. Now removing that, the test tube with standard reagent was kept in it and the optical density (OD) was found to be 0.40. The test tube was removed and the test tubes with the sample solution were kept in the calorie meter one after the other. The total phenolic content of both the samples were noted.Results and Discussion:-The preliminary phytochemical test indicates the presence of alkaloids, flavonoids, reducing sugars, terpenoids, and phyloblatannins in the lemon and orange seeds.It was also found that the optical density of orange seed was higher than that of the lemon seed. The optical density of the total protein reagent was found to be 0.40. The optical density of orange seed was determined to be 0.15 and the optical density of lemon seeds was determined to me 0.13. Total phenolic content was found to be:-Orange seeds: 36mg/gm of dry weight of extracts expressed as Gallic acid equivalent.Lemon seeds:- 33mg/gm of dry weight of extract expressed as Gallic acid equivalent.Phytochemical Analysis for orange seed:-PHYTOCHEMICAL ORANGE SEEDS OBSERVATION 1)Phylobatannins + Colourless2)Carbohydrates: a)Fehlings test + Green b)Benedicts test + Dark blue 3) Flavonoids + + + Colourless4) Alkaloids + + + Yellow5)Terpenoids + Light brown Phytochemical Analysis for Lemon seed :- PHYTOCHEMICAL LEMON SEEDS OBSERVATION 1)Phylobatannins + + Light red precipitate 2)Carbohydrates: a)Fehlings test + + Light red precipitate b)Benedicts test + Green 3) Flavonoids + + + Colourless 4) Alkaloids + + + Yellow 5)Terpenoids + Light brown Conclusion:-The preliminary phytochemical test indicates the presence of alkaloids, flavonoids, reducing sugars, terpenoids, and phyloblatannins in the lemon and orange seed extract. This further proves that citrus fruits can be used to help prevent deadly diseases such as cancer and heart attack. Phytochemicals are non-nutritive plant plant chemicals that have a protective or disease preventive properties. It is well known that plant produces these chemicals to protect themselves but it can also protect human beings against diseases. Presence of phenol in both the extracts reveal its antioxidant potential and its health benefits.